Transit Peptide Cleavage Sites of Integral Thylakoid Membrane Proteins
نویسندگان
چکیده
منابع مشابه
Toward the complete membrane proteome: high coverage of integral membrane proteins through transmembrane peptide detection.
To attain a comprehensive membrane proteome of two strains of Corynebacterium glutamicum (l-lysine-producing and the characterized model strains), both sample pretreatment and analysis methods were optimized. Isolated bacterial membranes were digested with trypsin/cyanogen bromide or trypsin/chymotrypsin, and a complementary protein set was identified using the multidimensional protein identifi...
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To attain a comprehensive membrane proteome of two strains of Corynebacterium glutamicum (L-lysine-producing and the characterized model strains), both sample pretreatment and analysis methods were optimized. Isolated bacterial membranes were digested with trypsin/ cyanogen bromide or trypsin/chymotrypsin, and a complementary protein set was identified using the multidimensional protein identif...
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With reference spectra derived from proteins of known structure (C h e n , Y a n g , and M a r t in e z , Biochemistry 11, 4120 [1972]) a better approximation o f the circular dichroism spectrum of fragments of the thylakoid membrane is achieved, than by the use of polylysine as reference substance. Most probably the protein in the thylakoid membrane consists of 40 per cent helix, 42 per cent r...
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Intact chloroplasts, purified from spinach leaves by sedimentation in density gradients of colloidal silica, incorporate labeled amino acids into at least 16 different polypeptides of the thylakoid membranes, using light as the only source of energy . The thylakoid products of chloroplast translation were visualized by subjecting membranes purified from chloroplasts labeled with [88S]methionine...
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The light reactions of photosynthesis in green plants are mediated by four large protein complexes, embedded in the thylakoid membrane of the chloroplast. Photosystem I (PSI) and Photosystem II (PSII) are both organized into large supercomplexes with variable amounts of membrane-bound peripheral antenna complexes. PSI consists of a monomeric core complex with single copies of four different LHC...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2003
ISSN: 1535-9476
DOI: 10.1074/mcp.m300062-mcp200